RNA-spin

RNA-spin Total RNA Extraction Kit (column type)

Cat. 17211 (50 columns) 17212 (100 columns)

Descriptions

RNA-spin Total RNA Extraction Kit is designed for rapid isolation of total RNA from cells, tissues, or bacteria. The purified RNA is ready for use such as cDNA synthesis, RT-PCR, Northern blot, dot blot, primer extension, et al . RNA-spin kit uses advanced silica-gel membrane technology for rapid and efficient purification of RNA without organic extraction or ethanol precipitation. Furthermore, the chaotropic salt in lysis buffer inactivates immediately RNase to ensure isolation of intact RNA. So, RNA-spin buffer system is optimized to allow rapid and simple cell lysis followed by selective binding of RNA to the column. Thus the purification procedure is less time consuming compared with alternative methods which require extraction with organic solution, RNA precipitation or ultracentrifugation.

RNA-spin procedure is very simple, so you can purify RNA from a variety of target source in less than 30 min.

Characteristics

1. High-quality total RNA in minutes
2. No phenol/chloroform extraction
3. No CsCl gradients, no ethanol precifitation
4. Ready-to-use RNA for any downstream application
5. High yield of RNA purification

Kit contents

1. R-buffer
   : Before use, must be add 10ml b-mercaptoethanol per 1ml R-buffer.
2. Washing buffer A
3. Washing buffer B
   : Washing buffer B is supplied as a concentrate.
   Before using for the first time, add 28ml (112ml) of absolute EtOH.
4. Elution buffer
5. Columns
   : Silica membrane based, polypropylene tube
6. Collection tube
   : polypropylene tube for 2ml