MEGA-spin

Cat. 17181 (50 columns) 17182 (250 columns)

Descriptions

DNA fragments for probe DNA or ligation must be separated and purified from other DNA fragments. MEGA-spin employs a column method to purify target DNA in excised agarose gel. The column method uses a highly concentrated salt solution to keep target DNA bound to the column membrane. Binding reaction occurs due to disruption of structures of water molecules and their interaction with nucleic acids.
Thus, the adsorption to the specifically pretreated membrane is favored. Since the binding process is specific for nucleic acids, the bound material can be separated and purified from impurities e.g. salts and proteins, by a simple washing step.
Nucleic acids elute from the column membrane in a low salt buffer or water. MEGA-spin is designed to extract and purify DNA of 100bp to 10kb from standard or low-melt agarose gels in TAE or TBE buffer. Furthermore, this kit promises a high yield of purification up to 70-90%.
DNA fragments isolated with MEGA-spin Agarose Gel Extraction Kit are efficiently ligated into plasmid cloning vectors or labeled to high specific activity using either random primed labeling or nick translation. No inhibition of digestion with restriction endonucleases is observed.

Applications

MEGA-spin Agarose Gel Extraction Kit is designed for efficient isolation of DNA fragments from TAE or TBE agarose gels. The kit is applicable for DNA isolation from standard agarose gels as well as low melting point agarose gels.

Characteristics

1) Compact designed silica-gel membrane
2) Fast DNA fragments extraction (10-15 minutes)
3) DNA cleanup in 3 simple steps: just bind, wash, and elute
4) Highly efficient recovery (80-95%) of a broad range of fragments(30 bp-30 kb)
5) Gel extraction from standard or low-melt agarose gels in TAE or TBE buffer
6) Efficient removal of contaminants
7) No resins, no phenol, no alcohol precipitation

Kit contents

1) Agarose lysis buffer
2) Washing buffer
: Add 40ml (80ml) of the absolute EtOH.
3) Elution buffer
4) MEGA-spin column
: Columns containing the membrane.
5) Collecting tube
: Polypropylene tube for 2ml.